![]() ![]() A 658-base pair sequence of COI (Cytochrome c Oxidase Subunit I) of 90 specimens was retrieved successfully, which confirmed the presence of 11 families, 25 genra and 47 species. To evaluate the authenticity of morphological identification, tissue samples of 96 specimens were sent to Canadian Center for Biodiversity and Genomics, University of Guelph, Canada. Morphological identification revealed the presence of 12 families, 29 genra and 49 species. Spiders were evaluated morphologically on the basis of different identification Keys and Catalogs. All the collected spiders were brought to molecular laboratory at GC University Lahore, preserved in 95% ethanol and stored at −20 ☌ until the DNA extraction. A total of 872 spiders were captured from June to August of 2017. In the present study, DNA barcoding was used to assess the percentage accuracy of morphological base identification of spiders from the agriculture fields of district Layyah, Punjab, Pakistan. It is concluded that DNA barcoding is a reliable tool to delimit studied spider species. Five species were also clearly separated based on parsimony analysis. The divergence values within and between species ranged from 0-1.6% and 17.6-23.7%, respectively. For every species, the maximum interaspecific divergence was less than the minimum distance to its nearest-neighbour. There was clear gap in intraspecific and interspecific values of genetic distances. DNA based data corresponded the morphological study, confirming the presence of five species i.e., Clubiona analis Thorell, 1895, Cyrtophora citricola (Forskal, 1775), Leucauge decorata (Blackwall, 1864), Neoscona theisi (Walckenaer, 1841) and Oxyopes javanus Thorell, 1887. The standard barcode region (658 base pairs of Cytochrome C oxidase subunit I), in multiple specimens of all species, was sequenced. Current study was designed to provide first barcode record of some common spiders from Punjab, Pakistan. DNA-barcoding is a promising approach to minimize the difficulties of taxonomists to delimit species. Spiders are important insect predators and being used as model organisms in various investigatory studies however, work on this group is difficult due to the constrains in their species level identification. ![]()
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